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KMID : 0604520050310010073
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Journal of the Society of Cosmetic Scientists of Korea
2005 Volume.31 No. 1 p.73 ~ p.78
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The Study for Antioxidative Effects of Acanthopanax sessiliflorus Extract as a New Cosmetics Ingredients Using Electron Paramagnetic Resonance
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Choi Shin-Wook
Kim Chang-Soo
Choi Myung-Soo
Kim Bo-Hyun
Kim Hak-Soo
Choi Dae-Sung
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Abstract
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This work was carried out to investigate the antioxidative effects of Acanthopnax sessiliflorus from Jeongseon County for the purpose of development of a novel antioxidant from natural products. The antioxidant activity was determined by using electron paramagnetic resonance (EPR), not measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) which have bun used for antioxidant activity of natural sources. Although DPPH radical scavenging activity assay have been generally used for antioxidant activity, this assay is nut appropriated for determinating which radical is scavenged by extracts from natural products. Using EPR, we determinated whether A. sessiliflorus extracts from Jeongseon County scavenge specific radicals or not. On experiment of scavenging superoxide anion radical, hydroxyl radical, nitrogen dioxide and peroxinitrite. Extracts from A. sessiliflorus showed high antioxidant activities to reactive oxygen and nitrogen species. These result suggest that extracts from A. sessiliflorus act as an antioxidant by scavenging reactive oxygen and nitrogen species and used as new cosmetic ingredients for anti-oxidative stress in skin.
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KEYWORD
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electron paramagnetic resonance, antioxidants, free radical, acanthopanax sessiliflorus, plant extracts
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